Dear colleague,
In 2018, the thrombin standardization group performed a survey on thrombin generation to make an inventory of the current (pre-analytical and analytical) practices used to measure thrombin generation.
The questionnaire consisted of 51 questions, and in total 240 replies were collected (of which 45% were complete). We would like to thank everybody that participated to our survey. The collected data was analyzed and published by de Laat-Kremers et al. in the Journal of Thrombosis and Haemostasis
(Romy M.W. de Laat-Kremers, Marisa Ninivaggi, Katrien M.J. Devreese, Bas de Laat. Towards standardization of thrombin generation assays: inventory of thrombin generation methods based on results of an ISTH SSC survey. J Thromb Haemost 2020; 18:1893-1899).
Following this publication, we formed a working group and provided recommendations for the standardized measurement of thrombin generation (Marisa Ninivaggi, Romy de Laat-Kremers, Armando Tripodi, Denis Wahl, Stephane Zuily, Yesim Dargaud, Hugo ten Cate, Vera Ignjatović, Katrien M.J. Devreese (equally last author), Bas de Laat. Recommendations for the Measurement of Thrombin Generation: Communication from the ISTH SSC Subcommittee on Lupus anticoagulant/Antiphospholipid Antibodies. J Thromb Haemost 2021;19: 1332-1378).
For the next step in our thrombin generation standardization project, we would like to ask the participation of various laboratories worldwide to test the standardized protocol in practice. For this purpose, we will distribute the standardized protocol, as well as 3 different lyophilized plasma samples that differ in coagulation potential. We ask to measure these samples according to the laboratory's in-house protocol and the recommended standardized protocol. We would also like to ask the laboratories to include their own normal pooled plasma, if available. All data will be collected, analyzed and published.
If you are interested in this project and would like to participate, please send an email to
m.ninivaggi@thrombin.com, and we will send more detailed information about the practical aspects of this study. We appreciate to receive your response before September 30th.
Thank you in advance on behalf of the Scientific and Standardization committee on LA/aPL and the thrombin generation standardization group,
Best regards,
Bas de Laat, Katrien Devreese, Marisa Ninivaggi
Principal investigators of the project